Journal: Experimental Biology and Medicine
Article Title: Featured Article: In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes
doi: 10.1177/1535370217728498
Figure Lengend Snippet: Characterization of isolated cells from cartilage tissues. Analysis of human chondrocytes in monolayer cultures at P2 (after ∼4 population doublings). Indirect immunofluorescence for collagen type II (a) and collagen type I (b) are shown. Red: antigens stained by Cy3 labeling; blue: cell nuclei stained with DAPI. Scale bars = 200 µm. (c) Relative gene expression levels of cartilage-specific markers (collagen type II, PRG4, S100B) and collagen type I normalized by β-actin expression. Here, three independent experiments were performed
Article Snippet: After washing three times with PBS, the slides were incubated for 1 h at RT with Cy3-conjugated goat anti-mouse (Type I and II Collagen) and goat anti-rabbit (S100) antibody (Dianova, Hamburg) diluted 1:600 in PBS/0.1% BSA including DAPI (1 µg/ml; Fluka, Seelze, Germany) to stain cell nuclei.
Techniques: Isolation, Immunofluorescence, Staining, Labeling, Expressing